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厌氧消化 3

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厌氧消化，生物固体，污染，甲基苯丙胺，吸附 1

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Evaluating the impact of sulfamethoxazole on hydrogen production during dark anaerobic sludge fermentation

《环境科学与工程前沿（英文）》 2023年 第17卷 第1期 doi: 10.1007/s11783-023-1607-2

摘要：

● SMX promotes hydrogen production from dark anaerobic sludge fermentation.

关键词： Sulfamethoxazole     Hydrogen production     Dark anaerobic fermentation     Waste activated sludge    

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Continuous biohydrogen production from diluted molasses in an anaerobic contact reactor

Sheng CHANG, Jianzheng LI, Feng LIU

《环境科学与工程前沿（英文）》 2011年 第5卷 第1期   页码 140-148 doi: 10.1007/s11783-010-0258-2

摘要： An anaerobic contact reactor (ACR) system comprising a continuous flow stirred tank reactor (CSTR) with settler to decouple the hydraulic retention time (HRT) from solids retention time (SRT) was developed for fermentative hydrogen production from diluted molasses by mixed microbial cultures. The ACR was operated at various volumetric loading rates (VLRs) of 20–44 kgCOD·m ·d with constant HRT of 6 h under mesophilic conditions of 35°C. The SRT was maintained at about 46–50 h in the system. At the initial VLR of 20 kgCOD·m ·d , the hydrogen production rate dropped from 22.6 to 1.58 L·d as the hydrogen was consumed by the hydrogentrophic methanogen. After increasing the VLR to 28 kgCOD·m ·d and discharging the sludge for 6 consecutive times, the hydrogentrophic methanogens were eliminated, and the hydrogen content reached 36.4%. As the VLR was increased to 44 kgCOD·m ·d , the hydrogen production rate and hydrogen yield increased to 42.1 L·d and 1.40 mol H ·molglucose-consumed , respectively. The results showed that a stable ethanol-type fermentation that favored hydrogen production in the reactor was thus established with the sludge loading rate (SLR) of 2.0–2.5 kgCOD·kgMLVSS ·d . It was found that the ethanol increased more than other liquid fermentation products, and the ethanol/acetic acid (mol/mol) ratio increased from 1.27 to 2.45 when the VLR increased from 28 to 44 kgCOD·m ·d , whereas the hydrogen composition decreased from 40.4% to 36.4%. The results suggested that the anaerobic contact reactor was a promising bioprocess for fermentative hydrogen production.

关键词： fermentative hydrogen production     anaerobic contact reactor (ACR)     sludge loading rate (SLR)     butyric acid-type fermentation     ethanol-type fermentation    

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High-solid anaerobic digestion of sewage sludge: achievements and perspectives

《环境科学与工程前沿（英文）》 2021年 第15卷 第4期 doi: 10.1007/s11783-020-1364-4

摘要：

• High-solid anaerobic digestion (HS-AD) of sewage sludge (SS) is overviewed.

关键词： High-solid effect     Anaerobic fermentation     Methane production     Biodegradability     Sludge treatment    

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Low-temperature caproate production, microbial diversity, and metabolic pathway in xylose anaerobic fermentation

《环境科学与工程前沿（英文）》 2023年 第17卷 第3期 doi: 10.1007/s11783-023-1637-9

摘要：

● Converting xylose to caproate under a low temperature of 20 °C by MCF was verified.

关键词： Xylose fermentation     Caproate     Low temperature     Bifidobacterium     FAB pathway     RBO pathway    

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Aerobic granular sludge formation based on substrate availability: Effects of flow pattern and fermentation

Quan Yuan, Hui Gong, Hao Xi, Kaijun Wang

《环境科学与工程前沿（英文）》 2020年 第14卷 第3期 doi: 10.1007/s11783-020-1226-0

摘要： Penetration depth and substrate characters affect AGS formation and performance. The relationship between substrate gradient and particle size affects AGS stability. The fermentation process is proposed as a pretreatment to improve AGS stability. The influences of flow patterns (mixed-flow and plug-flow) and fermentation pretreatment on aerobic granular sludge (AGS) formation with various substrate availability levels were investigated by running four identical laboratory-scale sequencing batch reactors (R1–R4), comparing two anaerobic feeding strategies and three kinds of substrates. R1 achieved faster granulation with a fast influent fill step followed by a modified anaerobic mixed-flow phase, but the AGS showed poorer stability with a cracked structure and a high suspended solids concentration in the effluent. The anaerobic plug-flow feeding mode (with influent fed slowly from the bottom) in R2 provided deeper penetration depth for the substance to reach the core of AGS and accordingly strengthen AGS stability. An acidogenic up-flow sludge bed reactor was introduced as a pretreatment to improve the AGS performance by enhancing glucose pre-fermentation (R4). AGS fed with mixed volatile fatty acids (VFA) after glucose fermentation showed similar performance compared with the reactor fed with acetate in the aspects of stability, structure, size distribution and nitrogen removal efficiency, and 74% similarity in the microbial community. For actual wastewater with low VFA concentrations, fermentation treatment was suggested as a promising pretreatment for stable AGS granulation and operation.

关键词： Anaerobic plug-flow feeding mode     Anaerobic mixed-flow mode     Fermentation pretreatment     Substrate gradient     Penetration depth    

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Effect of illumination on the hydrogen-production capability of anaerobic activated sludge

Guochen ZHENG, Jianzheng LI, Feng ZHAO, Liguo ZHANG, Li WEI, Qiaoying BAN, Yongsheng ZHAO

《环境科学与工程前沿（英文）》 2012年 第6卷 第1期   页码 125-130 doi: 10.1007/s11783-011-0384-5

摘要： To investigate the influence of illumination on the fermentative hydrogen production system, the hydrogen production efficiencies of two kinds of anaerobic activated sludge (floc and granule) from an anaerobic baffled reactor were detected under visible light, dark and light-dark, respectively. The 10 mL floc sludge or granular sludge was respectively inoculated to 100 mL diluted molasses (chemical oxygen demand of 8000 mg·L ) in a 250 mL serum bottle, and cultured for 24 h at 37°C under different illumination conditions. The results showed that the floc was more sensitive to illumination than the granule. A hydrogen yield of 19.8 mL was obtained in the dark with a specific hydrogen production rate of 3.52 mol·kg MLVSS·d (floc), which was the highest among the three illumination conditions. Under dark condition, the hydrogen yield of floc sludge reached the highest with the specific hydrogen production rate of 3.52 mol·kg MLVSS·d , and under light-dark, light, the specific hydrogen production rate was 3.11 and 2.21 mol·kg MLVSS·d , respectively. The results demonstrated that the illumination may affect the dehydrogenase activity of sludge as well as the activity of hydrogen-producing acetogens and then impact hydrogen production capacity.

关键词： biohydrogen production     dark fermentation     anaerobic activated sludge     light     dehydrogenase    

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Effect of the addition of organic carbon sources on nitrous oxide emission in anaerobic-aerobic (low

Hongjing LI, Xiurong CHEN, Yinguang CHEN

《环境科学与工程前沿（英文）》 2010年 第4卷 第4期   页码 490-499 doi: 10.1007/s11783-010-0248-4

摘要： The effect of additional organic carbon sources on the production of nitrous oxide (N O) in anaerobic-aerobic (low dissolved oxygen) real wastewater treatment system was investigated. In this paper, three laboratory-scale sequencing batch reactors (SBRs) (SBR-1, SBR-2 and SBR-3) were operating under an anaerobic-aerobic (low dissolved oxygen, 0.15–0.45 mg·L ) configuration. The SBRs were ‘long-term cultured’ respectively with a single municipal wastewater sample, sodium acetate, and a waste-activated sludge alkaline fermentation liquid as the additional carbon sources of real wastewater. Off-gas analysis showed that N O was emitted into the atmosphere during the aerobic (low dissolved oxygen) period in the three SBRs, and the order of N O emission rate was SBR-2>SBR-1>SBR-3. It was observed that the higher poly-β-hydroxyvalerate fraction of polyhydroxyalkanoates, the lower glycogen transformation and less nitrite accumulation was in SBR-3, while the opposite behavior was observed in SBR-2. Further research indicated that the interaction of the factors above potentially affected the N O emission in the anaerobic-aerobic (low dissolved oxygen) system.

关键词： anaerobic-aerobic (low dissolved oxygen)     nitrous oxide (N2O) emitting rate     municipal wastewater     waste activated sludge     alkaline fermentation liquid    

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Effect of different gas releasing methods on anaerobic fermentative hydrogen production in batch cultures

Sheng CHANG, Jianzheng LI, Feng LIU, Ze YU

《环境科学与工程前沿（英文）》 2012年 第6卷 第6期   页码 901-906 doi: 10.1007/s11783-012-0403-1

摘要： Decreasing hydrogen partial pressure can not only increase the activity of the hydrogen enzyme but also decrease the products inhibition, so it is an appropriate method to enhance the fermentative hydrogen production from anaerobic mixed culture. The effect of biogas release method on anaerobic fermentative hydrogen production in batch culture system was compared, i.e., Owen method with intermediately release, continuous releasing method, and continuous releasing+ CO absorbing. The experimental results showed that, at 35°C, initial pH 7.0 and glucose concentration of 10 g·L , the hydrogen production was only 28 mL when releasing gas by Owen method, while it increased two times when releasing the biogas continuously. The cumulative hydrogen production could reach 155 mL when carbon dioxide in the gas stream was continuously absorbed by 1 mol·L NaOH. The results showed that acetate was dominated, accounting for 43% in the dissolved fermentation products in Owen method, whereas the butyrate predominated and reached 47%–53% of the total liquid end products when releasing gas continuously. It is concluded that the homoacetogenesis could be suppressed when absorbing CO in the gas phase in fermentative hydrogen production system.

关键词： batch fermentation     hydrogen production     biogas releasing     hydrogen pressure     homoacetogenesis    

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production from beet sugar factory wastewater treatment in a continuous stirred tank reactor using anaerobic

Gefu ZHU, Chaoxiang LIU, Jianzheng LI, Nanqi REN, Lin LIU, Xu HUANG

《环境科学与工程前沿（英文）》 2013年 第7卷 第1期   页码 143-150 doi: 10.1007/s11783-012-0456-1

摘要： A low pH, ethanol-type fermentation process was evaluated for wastewater treatment and bio-hydrogen production from acidic beet sugar factory wastewater in a continuous stirred tank reactor (CSTR) with an effective volume of 9.6 L by anaerobic mixed cultures in this present study. After inoculating with aerobic activated sludge and operating at organic loading rate (OLR) of 12 kgCOD?m ·d , HRT of 8h, and temperature of 35°C for 28 days, the CSTR achieved stable ethanol-type fermentation. When OLR was further increased to 18 kgCOD?m ·d on the 53rd day, ethanol-type fermentation dominant microflora was enhanced. The liquid fermentation products, including volatile fatty acids (VFAs) and ethanol, stabilized at 1493 mg·L in the bioreactor. Effluent pH, oxidation-reduction potential (ORP), and alkalinity ranged at 4.1–4.5, -250–(-290) mV, and 230–260 mgCaCO ?L . The specific hydrogen production rate of anaerobic activated sludge was 0.1 L?gMLVSS ·d and the COD removal efficiency was 45%. The experimental results showed that the CSTR system had good operation stability and microbial activity, which led to high substrate conversion rate and hydrogen production ability.

关键词： fermentative hydrogen production     continuous stirred tank reactor (CSTR)     specific hydrogen production rate     beet sugar factory wastewater     ethanol-type fermentation    

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市政污泥热水解—真菌发酵产菌丝纤维回收有机质资源的研究 Article

梁嘉晋, 李炳, 温磊, 李若泓, 李晓岩

《工程（英文）》 2021年 第7卷 第2期   页码 203-211 doi: 10.1016/j.eng.2020.09.002

摘要：

市政污泥的处理已成为许多大城市的环境治理难题。本研究提出了基于热水解—真菌发酵—厌氧消化的三阶段精炼策略，旨在实现市政污泥的减量化和资源化。在市政污泥热水解处理中，当热水解温度由140 ℃升至180 ℃时，可以显著地提升市政污泥的减量效果和有机物的释放效率（p < 0.05）。市政污泥经过140 ℃、160 ℃和180 ℃两级热水解处理后，总挥发性固体（TVS）的去除率分别为36.6%、47.7%和58.5%，总有机碳（TOC）的溶解释放效率分别达到28.0%、38.0%和45.1%。在160 ℃下，污泥热水解上清液中的多糖和蛋白质的含量最为丰富，而在180 ℃时，由于在热水解过程中发生了美拉德反应，污泥热水解上清液的腐殖酸类物质的含量显著增加（p < 0.05）。采用黑曲霉（Aspergillus niger）进行真菌发酵，可以将市政污泥热水解上清液中的有机物转化为高附加值的菌丝纤维。在140 ℃和160 ℃下，污泥热水解上清液经过真菌发酵后，菌丝纤维的生物量分别达到1.30 g·L–1和1.27 g·L–1，对应的有机物转化率为24.6%和24.0%。从污泥热水解上清液真菌中回收的菌丝纤维可以用于生产纸基材料等高附加值产品。菌丝纤维纸结构致密，具有较好的力学性能，抗张强度可以达到10.75 N·m·g–1。在160 ℃下，污泥热水解上清液经真菌发酵耦合厌氧消化两级生物处理，能将热水解上清液超过75%的有机物进行综合利用以回收菌丝纤维和沼气。

关键词： 市政污泥     热水解     真菌发酵     菌丝纤维     厌氧消化     污泥处理    

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Simultaneous saccharification and fermentation of wheat bran flour into ethanol using coculture of amylotic

K. Manikandan, T. Viruthagiri

《化学科学与工程前沿（英文）》 2009年 第3卷 第3期   页码 240-249 doi: 10.1007/s11705-009-0205-6

摘要： Studies on simultaneous saccharification and fermentation (SSF) of wheat bran flour, a grain milling residue as the substrate using coculture method were carried out with strains of starch digesting and nonstarch digesting and sugar fermenting in batch fermentation. Experiments based on central composite design (CCD) were conducted to maximize the glucose yield and to study the effects of substrate concentration, pH, temperature, and enzyme concentration on percentage conversion of wheat bran flour starch to glucose by treatment with fungal α-amylase and the above parameters were optimized using response surface methodology (RSM). The optimum values of substrate concentration, pH, temperature, and enzyme concentration were found to be 200 g/L, 5.5, 65°C and 7.5 IU, respectively, in the starch saccharification step. The effects of pH, temperature and substrate concentration on ethanol concentration, biomass and reducing sugar concentration were also investigated. The optimum temperature and pH were found to be 30°C and 5.5, respectively. The wheat bran flour solution equivalent to 6% ( / ) initial starch concentration gave the highest ethanol concentration of 23.1 g/L after 48 h of fermentation at optimum conditions of pH and temperature. The growth kinetics was modeled using Monod model and Logistic model and product formation kinetics using Leudeking-Piret model. Simultaneous saccharificiation and fermentation of liquefied wheat bran starch to bioethanol was studied using coculture of amylolytic fungus and nonamylolytic sugar fermenting .

关键词： simultaneous saccharification and fermentation (SSF)     starch     coculture fermentation     statistical experimental design     bioethanol     Monod model    

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The role of lipids in fermentative propionate production from the co-fermentation of lipid and food waste

《环境科学与工程前沿（英文）》 2023年 第17卷 第7期 doi: 10.1007/s11783-023-1686-0

摘要：

● Lipid can promote PA production on a target from food waste.

关键词： Acidogenic fermentation     Microbial community     Volatile fatty acid     Propionate     Food waste     Lipid    

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Repeated batch fermentation with water recycling and cell separation for microbial lipid production

Yumei WANG, Wei LIU, Jie BAO

《化学科学与工程前沿（英文）》 2012年 第6卷 第4期   页码 453-460 doi: 10.1007/s11705-012-1210-8

摘要： Large waste water disposal was the major problem in microbial lipid fermentation because of low yield of lipid. In this study, the repeated batch fermentation was investigated for reducing waste water generated in the lipid fermentation of an oleaginous yeast CX1 strain. The waste fermentation broth was recycled in the next batch operation after the cells were separated using two different methods, centrifugation and flocculation. Two different sugar substrates, glucose and inulin, were applied to the proposed operation. The result showed that at least 70% of the waste water was reduced, while lipid production maintained satisfactory in the initial four cycles. Furthermore, it is suggested that CX1 cells might produce certain naturally occurring inulin hydrolyzing enzyme(s) for obtaining fructose and glucose from inulin directly. Our method provided a practical option for reducing the waste water generated from microbial lipid fermentation.

关键词： batch fermentation     microbial lipid     Trichosporon cutaneum CX1     flocculation     waste water recycle    

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Simultaneous saccharification and fermentation of sweet potato powder for the production of ethanol under

Yinxiu CAO, Hongchi TIAN, Kun YAO, Yingjin YUAN

《化学科学与工程前沿（英文）》 2011年 第5卷 第3期   页码 318-324 doi: 10.1007/s11705-010-1026-3

摘要： Due to its merits of drought tolerance and high yield, sweet potatoes are widely considered as a potential alterative feedstock for bioethanol production. Very high gravity (VHG) technology is an effective strategy for improving the efficiency of ethanol fermentation from starch materials. However, this technology has rarely been applied to sweet potatoes because of the high viscosity of their liquid mash. To overcome this problem, cellulase was added to reduce the high viscosity, and the optimal dosage and treatment time were 8 U/g (sweet potato powder) and 1 h, respectively. After pretreatment by cellulase, the viscosity of the VHG sweet potato mash (containing 284.2 g/L of carbohydrates) was reduced by 81%. After liquefaction and simultaneous saccharification and fermentation (SSF), the final ethanol concentration reached 15.5% (v/v), and the total sugar conversion and ethanol yields were 96.5% and 87.8%, respectively.

关键词： bioethanol     sweet potato     very high gravity     viscosity reduction     simultaneous saccharification and fermentation    

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Biological hydrogen production from organic wastewater by dark fermentation in China: Overview and prospects

Nanqi REN, Wanqian GUO, Bingfeng LIU, Guangli CAO, Jing TANG

《环境科学与工程前沿（英文）》 2009年 第3卷 第4期   页码 375-379 doi: 10.1007/s11783-009-0148-7

摘要： Biological hydrogen production by dark fermentation is an important part of biological hydrogen production technologies. China is a typical developing country that heavily relies on fossil fuels; thus, new, clean, and sustainable energy development turns quite urgent. It is delightful that Chinese government has already drawn up several H2 development policies since 1990s and provided financial aid to launch some H development projects. In this paper, the research status on dark fermentative hydrogen production in China was summarized and analyzed. Subsequently, several new findings and achievements, with some of which transformed into scale-up tests, were highlighted. Moreover, some prospecting coupling processes with dark fermentation of hydrogen production were also proposed to attract more research interests in the future.

关键词： biological hydrogen production     dark fermentation     overview     prospects    

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标题 作者 时间 类型 操作

Evaluating the impact of sulfamethoxazole on hydrogen production during dark anaerobic sludge fermentation

期刊论文

Continuous biohydrogen production from diluted molasses in an anaerobic contact reactor

Sheng CHANG, Jianzheng LI, Feng LIU

期刊论文

High-solid anaerobic digestion of sewage sludge: achievements and perspectives

期刊论文

Low-temperature caproate production, microbial diversity, and metabolic pathway in xylose anaerobic fermentation

期刊论文

Aerobic granular sludge formation based on substrate availability: Effects of flow pattern and fermentation

Quan Yuan, Hui Gong, Hao Xi, Kaijun Wang

期刊论文

Effect of illumination on the hydrogen-production capability of anaerobic activated sludge

Guochen ZHENG, Jianzheng LI, Feng ZHAO, Liguo ZHANG, Li WEI, Qiaoying BAN, Yongsheng ZHAO

期刊论文

Effect of the addition of organic carbon sources on nitrous oxide emission in anaerobic-aerobic (low

Hongjing LI, Xiurong CHEN, Yinguang CHEN

期刊论文

Effect of different gas releasing methods on anaerobic fermentative hydrogen production in batch cultures

Sheng CHANG, Jianzheng LI, Feng LIU, Ze YU

期刊论文

production from beet sugar factory wastewater treatment in a continuous stirred tank reactor using anaerobic

Gefu ZHU, Chaoxiang LIU, Jianzheng LI, Nanqi REN, Lin LIU, Xu HUANG

期刊论文

市政污泥热水解—真菌发酵产菌丝纤维回收有机质资源的研究

梁嘉晋, 李炳, 温磊, 李若泓, 李晓岩

期刊论文

Simultaneous saccharification and fermentation of wheat bran flour into ethanol using coculture of amylotic

K. Manikandan, T. Viruthagiri

期刊论文

The role of lipids in fermentative propionate production from the co-fermentation of lipid and food waste

期刊论文

Repeated batch fermentation with water recycling and cell separation for microbial lipid production

Yumei WANG, Wei LIU, Jie BAO

期刊论文

Simultaneous saccharification and fermentation of sweet potato powder for the production of ethanol under

Yinxiu CAO, Hongchi TIAN, Kun YAO, Yingjin YUAN

期刊论文

Biological hydrogen production from organic wastewater by dark fermentation in China: Overview and prospects

Nanqi REN, Wanqian GUO, Bingfeng LIU, Guangli CAO, Jing TANG

期刊论文